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Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
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Chapters
- 00:05Introduction
- 00:44Harvesting Donor Rat Mammary Gland Epithelium
- 01:35Extract Brain Tissue from Euthanized Donors
- 03:04Digestion and Processing of Mammary Gland Extracts
- 05:08Transplantation Procedure (Recipient Rat 4-5 Weeks of Age)
- 06:53Assessment of Epithelial Outgrowth
- 07:40Results: Cell Transplantation into the Interscapular White Fat Pad
- 09:04Conclusion
This article describes a transplantation method to graft donor rat mammary epithelial cells into the interscapular white fat pad of recipient animals. This method can be used to examine host and/or donor effects on mammary epithelium development and eliminates the need for pre-clearing, thereby extending the usefulness of this technique.
