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Estimativa estereológica do comprimento da fibra cholinergica no Núcleo Basalis de Meynert do Cérebro do Rato
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1Laboratory for Alzheimer’s Disease and Aging Research,Kansas City Veterans Affairs Medical Center, 2Department of Neurology,University of Kansas Medical Center, 3Department of Molecular and Integrative Physiology,University of Kansas Medical Center, 4The University of Kansas Alzheimer’s Disease Center
Chapters
- 00:04Introduction
- 01:09Perfusion and Tissue Processing
- 02:45Systematic Section Selection
- 03:19Immunohistochemistry (IHC)
- 04:46Stereology
- 08:10Results: Representative Mouse Brain Cholinergic Fiber Length Estimation
- 08:38Conclusion
O comprimento da fibra neuronal dentro de uma estrutura tridimensional de uma região cerebral é um parâmetro confiável para quantificar a integridade estrutural neuronal específica ou a degeneração. Este artigo detalha um método de quantificação estereológica para medir o comprimento da fibra cholinergicdentro do núcleo basalis de Meynert em camundongos como exemplo.
Tags
Keywords:
Stereological EstimationCholinergic Fiber LengthNucleus Basalis Of MeynertMouse BrainNeurodegenerationNeuronal Fiber LossCell Body LossStereological QuantificationUnbiased FractionationLine-like StructuresNeuronal FibersTranscardial PerfusionDPBSPFASucroseCryotomeSectioningImmunohistochemical LabelingNormal Bovine SerumTriton X-100Tris-buffered Saline
