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Measuring Microbial Mutation Rates with the Fluctuation Assay
JoVE Journal
Genetics
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JoVE Journal Genetics
Measuring Microbial Mutation Rates with the Fluctuation Assay

Measuring Microbial Mutation Rates with the Fluctuation Assay

DOI:
10.3791/60406-v

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07:44 min

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November 28, 2019

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, , , , ,
1School of Biological Sciences,The University of Manchester, 2School of Science,University of Waikato, 3School of Natural Sciences,The University of Manchester, 4Department of Mathematics,Université du Québec à Montréal

Chapters

  • 00:05Title
  • 01:09Day 1: Inoculation and Acclimation of Cultures
  • 01:49Day 2: Generation of Mutants in Parallel Cultures
  • 03:27Day 3: Plating Cultures on Selective and Non-selective Agar Plates
  • 04:48Day 4 and Day 5
  • 05:48Results: Analysis of the Fluctuation Assay
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.

Tags

Keywords: Microbial Mutation RatesFluctuation AssayAntibiotic ResistanceAntimicrobial ResistanceMonocultureCo-cultureE. Coli K12Davis Minimal MediumGlucoseOptical DensityInoculumDeep Well PlateIncubationEvaporation

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