A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:12Preparation of P. aeruginosa PAO1 Culture
- 01:58Preparation of Nematode Growth Medium (NGM) Plates for Testing the Effects of Bacteria and DIM on the Intestinal Permeability of C. elegans
- 02:57Bacteria or DIM Treatment and FITC-Dextran Feeding
- 04:19Imaging of C. elegans with the Operetta Imaging System and Determination of Intestinal Permeability
- 05:43Results: Effects of Bacteria on Intestinal Permeability of P. aeruginosa
- 06:40Conclusion
This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases.
