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Journal / Immunology and Infection / Analys av grupp IV viral SSHHPS med in vitro-och…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Analys av grupp IV viral SSHHPS med in vitro-och silico-metoder
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Analys av grupp IV viral SSHHPS med in vitro-och silico-metoder

Article DOI: 10.3791/60421-v • 10:40 min • December 21st, 2019
December 21st, 2019 •
1, 2, 2
1National Center for Advancing Translational Sciences, National Institutes of Health, 2United States Naval Research Laboratory

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Vi presenterar ett allmänt protokoll för att identifiera korta sträckor av homolog värd-patogen proteinsekvenser (SSHHPS) inbäddade i viral polyprotein. SSHHPS erkänns av virala proteaser och rikta den riktade förstörelsen av specifika värd proteiner av flera grupp IV virus.

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Immunologi och infektion enzym assay FRET icke-strukturellt protein alphavirus grupp IV proteas SSHHPS gel assay in vitro dockning
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