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Journal / Neuroscience / Isolamento e Cultura dos neurônios oculomotores,…
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JoVE Journal Neuroscience
Isolamento e Cultura dos neurônios oculomotores, trochlear e motores espinhais de pré-natal Islmn
Micro-Drive System Assembly and Electrode Array Implantation for Multichannel Recording in Mice (Video) | JoVE
Journal (Neuroscience)
Micro-Drive System Assembly and Electrode Array Implantation for Multichannel Recording in Mice (Video) | JoVE
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
Journal (Neuroscience)
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
 
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Isolamento e Cultura dos neurônios oculomotores, trochlear e motores espinhais de pré-natal Islmn:GFP Ratos Transgênicos

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Este trabalho apresenta um protocolo para produzir culturas celulares homogêneas de neurônios motores oculomotores primários, trochlear e espinhais. Essas culturas podem ser usadas para análises comparativas das características morfológicas, celulares, moleculares e eletrofisiológicas dos neurônios motores oculares e espinhais.

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Neurociência Edição 153 neurônio motor neurônio oculomotor neurônio trochlear cultura primária cultura embrionária do neurônio motor do rato FACS IslMN:GFP mouse transgênico purificação celular isolamento celular
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