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JoVE Journal
Cancer Research

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Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
 

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Article DOI: 10.3791/60469-v 08:43 min January 31st, 2020
January 31st, 2020

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Summary

This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.

Tags

Keywords: Tumor Organoids Bladder Cancer Cell Culture Genetic Manipulation Orthotopic Transplantation Tumor Microenvironment
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