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Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
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Chapters
- 00:05Introduction
- 00:52Left Anterior Descending Artery (LAD) Ligation-Induced Coronary Artery Occlusion (Myocardial Infarction)
- 02:58Passive Clear Lipid-Exchanged Acrylamide-Hybridized Rigid Imaging Compatible Tissue Hydrogel (CLARITY)
- 05:53Single-Photon Confocal Microscopy Cleared Mouse Heart Imaging
- 06:28Results: Representative Neonatal Mouse Visualization
- 07:46Conclusion
Cardiomyocyte proliferation following injury is a dynamic process that requires a symphony of extracellular cues from non-myocyte cell populations. Utilizing lineage tracing, passive CLARITY, and three-dimensional whole-mount confocal microscopy techniques, we can analyze the influence of a variety of cell types on cardiac repair and regeneration.
