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Optagelse af hjerteskaderespons af målrettede cellepopulationer via clearet hjerte tredimensionel billeddannelse
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
DOI:

08:14 min

March 17, 2020

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Chapters

  • 00:05Introduction
  • 00:52Left Anterior Descending Artery (LAD) Ligation-Induced Coronary Artery Occlusion (Myocardial Infarction)
  • 02:58Passive Clear Lipid-Exchanged Acrylamide-Hybridized Rigid Imaging Compatible Tissue Hydrogel (CLARITY)
  • 05:53Single-Photon Confocal Microscopy Cleared Mouse Heart Imaging
  • 06:28Results: Representative Neonatal Mouse Visualization
  • 07:46Conclusion

Summary

Automatic Translation

Cardiomyocyt spredning efter skade er en dynamisk proces, der kræver en symfoni af ekstracellulære signaler fra ikke-myocyte cellepopulationer. Ved hjælp af afstamning sporing, passiv KLARHED, og tre-dimensionelle hel-mount konfokal mikroskopi teknikker, kan vi analysere indflydelsen af en række forskellige celletyper på hjerte reparation og regenerering.

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