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Quantification of Proliferative and Dead Cells in Enteroids
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JoVE Journal Biology
Quantification of Proliferative and Dead Cells in Enteroids

Quantification of Proliferative and Dead Cells in Enteroids

DOI:
10.3791/60501-v

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10:47 min

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January 31, 2020

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, , , , , , , , , , ,
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology,The First Affiliated Hospital of Soochow University, 2Department of Pathology,University of Chicago, 3Department of Pathology,Brigham and Women’s Hospital–Harvard Medical School

Chapters

  • 00:05Introduction
  • 01:00Intestinal Organoid Isolation and Culture
  • 04:18Flow Cytometry Analysis of EdU-Positive Cells in Enteroids
  • 08:40Results: IL-22 Increases Enteroid Proliferation and Promotes Cell Death in Enteroids
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.

Tags

Keywords: EnteroidProliferationCell DeathIntestinal Epithelial CellsInflammatory Bowel DiseaseOrganoidIntestinal CryptBasement Membrane Matrix

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