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1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology,The First Affiliated Hospital of Soochow University, 2Department of Pathology,University of Chicago, 3Department of Pathology,Brigham and Women’s Hospital–Harvard Medical School
Chapters
- 00:05Introduction
- 01:00Intestinal Organoid Isolation and Culture
- 04:18Flow Cytometry Analysis of EdU-Positive Cells in Enteroids
- 08:40Results: IL-22 Increases Enteroid Proliferation and Promotes Cell Death in Enteroids
- 09:46Conclusion
O protocolo apresentado utiliza citometria de fluxo para quantificar o número de células proliferantes e mortas em enteróides de camundongos cultivados. Este método é útil para avaliar os efeitos do tratamento medicamentoso sobre a proliferação organoide e a sobrevivência.
