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JoVE Journal
Developmental Biology

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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
 

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Article DOI: 10.3791/60503-v
December 18th, 2019

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Summary December 18th, 2019

This protocol describes laser capture microdissection for the isolation of cartilage and bone from fresh frozen sections of the mouse embryo. Cartilage and bone can be rapidly visualized by cresyl violet staining and collected precisely to yield high quality RNA for transcriptomic analysis.

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