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Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss
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JoVE Journal Medicine
Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

DOI:
10.3791/60542-v

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06:23 min

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April 24, 2020

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, , , , , , , , ,
1Laboratory for Angiogenesis and Ocular Cell Transplantation,University of Lübeck, 2Department of Ophthalmology,University of Lübeck, 3Institute of Biomedical Optics,University of Lübeck

Chapters

  • 00:04Introduction
  • 00:44Corneal Tissue Laser Treatment and Culture
  • 02:46Histology Preparation
  • 03:45Hematoxylin and Eosin (H&E) Staining
  • 05:02Results: Representative Probability of Selective Corneal Endothelial Cell (CEC) Damage
  • 05:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to detach corneal endothelial cells (CEC) from Descemet’s membrane (DM) using a neodymium:YAG (Nd:YAG) laser as an ex vivo disease model for bullous keratopathy (BK).

Tags

Keywords: Corneal Endothelial Cell LossNoninvasive ModelLaser-assistedNd:YAG LaserOptical Coherence TomographyCorneal LayersParacentesisTrephineParaformaldehydeCryostat Sectioning

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