Journal
/
Developmental Biology
/
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

DOI:
10.3791/60550-v

•

07:30 min

•

December 07, 2019

•

,
1Photonics Group, Department of Physics,Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Chapters

  • 00:04Title
  • 00:29Differential Fluorescent Cell Labeling
  • 02:00Cell-Cell Fusion
  • 03:03Fused Cell Enrichment
  • 04:18Cell-Cell Fusion Immunofluorescent Staining and Imaging
  • 05:30Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

Tags

Keywords: Cell-cell FusionGenome EditingCellular StructureCellular FunctionFluorescent LabelingDye LabelingCell CultureCell FusionPolyethylene Glycol (PEG)Cell ImagingOrganelle HomeostasisOrganelle Function

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60550?article_type=v

Method of Studying Palatal Fusion using Static Organ Culture
check_url/60550?article_type=v

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
check_url/60550?article_type=v

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
check_url/60550?article_type=v

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
check_url/60550?article_type=v

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
check_url/60550?article_type=v

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
check_url/60550?article_type=v

Live Imaging of Mouse Secondary Palate Fusion
check_url/60550?article_type=v

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
check_url/60550?article_type=v

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
check_url/60550?article_type=v

Live Cell Imaging of Chromosome Segregation During Mitosis

Read Article