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Journal / Developmental Biology / Celle celle fusion af Genomredigerede cellelinjer …
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Developmental Biology

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JoVE Journal Developmental Biology
Celle celle fusion af Genomredigerede cellelinjer til Perturbation af cellulær struktur og funktion
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Celle celle fusion af Genomredigerede cellelinjer til Perturbation af cellulær struktur og funktion

Article DOI: 10.3791/60550-v • 07:30 min • December 7th, 2019
December 7th, 2019 •
1,2, 3
1Photonics Group, Department of Physics, Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus, University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus, University of Cambridge

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Formålet med denne protokol er at smelte to forskellige celletyper for at oprette hybridceller. Fluorescens mikroskopi analyse af smeltet celler bruges til at spore cellen af oprindelsen af cellulære organeller. Denne analyse kan bruges til at undersøge, hvordan cellulær struktur og funktion reagerer på forstyrrelse ved cellefusion.

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Udviklingsmæssige biologi celle-cellefusion centrosome euploidy heterokaryon flow cytometry polyethylenglycol (PEG) Fluorescens mikroskopi organelle fusogen
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