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JoVE Journal
Bioengineering

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Generation of Transgenic Rats using a Lentiviral Vector Approach
 

Generation of Transgenic Rats using a Lentiviral Vector Approach

Article doi: 10.3791/60570
May 17th, 2020

Summary May 17th, 2020

This article aims to provide the methodology for lentiviral transgenesis in rat embryos using multiple injections of a virus suspension into the zygote perivitelline space. Female rats that are mated with a fertile male strain with a different dominant fur color is used to generate pseudopregnant foster mothers.

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