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Generation of Transgenic Rats using a Lentiviral Vector Approach
Generation of Transgenic Rats using a Lentiviral Vector Approach
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Transgenic Rats using a Lentiviral Vector Approach

Generation of Transgenic Rats using a Lentiviral Vector Approach

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09:07 min

May 17, 2020

DOI:

09:07 min
May 17, 2020

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Summary

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This article aims to provide the methodology for lentiviral transgenesis in rat embryos using multiple injections of a virus suspension into the zygote perivitelline space. Female rats that are mated with a fertile male strain with a different dominant fur color is used to generate pseudopregnant foster mothers.

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