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March 27, 2020
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Summary
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To identify novel regulators of transcription factors, we developed an approach to screen arrayed lentiviral or retroviral RNAi libraries using a dual-luciferase-based transcriptional reporter assay. This approach offers a quick and relatively inexpensive way to screen hundreds of candidates in a single experiment.
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Cite this Article
Xiao, Y., Lamar, J. M. Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter. J. Vis. Exp. (157), e60582, doi:10.3791/60582 (2020).
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