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A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

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Immunology and Infection

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JoVE Journal Immunology and Infection

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

DOI:

10.3791/60599-v

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06:56 min

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February 20, 2020

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Christiaan R. de Vries*¹, Johanna M. Sweere*^1,2, Heather Ishak³, Vivekananda Sunkari, Michelle S. Bach, Dan Liu, Robert Manasherob, Paul L. Bollyky²

¹Division of Infectious Diseases, School of Medicine,Stanford University, ²Stanford Immunology,Stanford University, ³Palo Alto Veterans Institute of Research

Chapters

00:05Introduction
00:50Hair Removal
01:28Full Thickness Excisional Wound Surgery
02:51P. aeruginosa Inoculation
03:57In vivo Infected Wound Imaging
04:39Wound Excision
05:20Results: Representative P. aeruginosa Wound Infection Detection
06:22Conclusion

Summary

Automatic Translation

We describe a delayed inoculation protocol for generating chronic wound infections in immunocompetent mice.

Tags

Keywords: Chronic Pseudomonas Aeruginosa Wound Infection Delayed Inoculation Model Full Thickness Excisional Wounding Host-pathogen Interactions Bacterial Pathogenesis New Therapies Mouse Model Skin Biopsy Punch Excisional Wounds Transparent Film Dressing

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