Perfusion and Inflation of the Mouse Lung for Tumor Histology

Mackenzie  L. Davenport; Taylor  P. Sherrill; Timothy  S. Blackwell; Mick D. Edmonds

doi:10.3791/60605

Perfusion and Inflation of the Mouse Lung for Tumor Histology

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Perfusion and Inflation of the Mouse Lung for Tumor Histology

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04:24 min

August 6, 2020

DOI: 10.3791/60605-v

Mackenzie L. Davenport¹, Taylor P. Sherrill², Timothy S. Blackwell², Mick D. Edmonds¹

¹Department of Genetics,University of Alabama at Birmingham, ²Department of Medicine, Division of Allergy, Pulmonary, and Critical Care Medicine,Vanderbilt University School of Medicine

Overview

This article presents a straightforward method for the perfusion, inflation, and fixation of mouse lungs. This technique is essential for examining lung tumor pathology and evaluating metastases to the lung.

Key Study Components

Area of Science

Neuroscience
Pathology
Oncology

Background

Understanding lung tumor pathology is crucial for cancer research.
Mouse models are commonly used to study human diseases.
Effective fixation techniques are necessary for accurate histological analysis.
This method allows for detailed examination of lung tissues.

Purpose of Study

To provide a reliable method for lung perfusion and fixation.
To facilitate the study of lung tumors and metastases.
To improve the accuracy of pathological evaluations.

Methods Used

Euthanizing the mouse prior to the procedure.
Making incisions to access the abdominal cavity and thorax.
Utilizing surgical scissors for precise cuts.
Inspecting axillary lymph nodes during the procedure.

Main Results

The method allows for effective lung inflation and fixation.
Pathological examination of lung tissues is enhanced.
Metastatic evaluation becomes more reliable.
Demonstrated by a graduate student in a laboratory setting.

Conclusions

This method is a valuable tool for lung cancer research.
It provides insights into tumor pathology and metastasis.
Future studies can build upon this technique for further discoveries.

Frequently Asked Questions

What is the significance of lung perfusion in research?

Lung perfusion is crucial for studying tumor pathology and understanding metastases.

How does this method improve pathological evaluations?

It allows for better fixation and preservation of lung tissues, leading to more accurate analyses.

Who demonstrated the procedure in this article?

Mackenzie Davenport, a graduate student from the Edmonds Laboratory, demonstrated the procedure.

What tools are used in this method?

Surgical scissors are primarily used for making incisions during the procedure.

Can this method be applied to other animal models?

While this method is designed for mouse lungs, similar techniques may be adapted for other models.

What are the potential applications of this research?

This research can aid in understanding lung cancer and developing new therapeutic strategies.

The purpose of this method is to present a simple and efficient method for the perfusion, inflation, and fixation of mouse lungs for the examination of lung tumor pathology and evaluation of metastases to the lung.

Demonstrating the procedure will be Mackenzie Davenport, a graduate student from the Edmonds Laboratory. After euthanizing the mouse, use surgical scissors to make a 3.5 to five millimeter horizontal incision in the middle of the lower abdomen. Next, insert surgical scissors into the incision and cut vertically up the center midline to just below the neck of the mouse.

Pull the skin back with fingers and inspect the axillary lymph nodes. Use surgical scissors to make a 3.5 millimeter lateral incision to open the abdominal cavity. Then cut in the interior direction up to the bottom of the thorax.

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