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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

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04:45 min

February 10, 2022

DOI:

04:45 min
February 10, 2022

6758 Views
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Summary

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Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.

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