Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Robert P. Sparks; William Lawless; Andres  S. Arango; Emad Tajkhorshid; Rutilio  A. Fratti

doi:10.3791/60607

Journal

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Biochemistry

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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

6,778 Views

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04:45 min

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February 10, 2022

DOI:

10.3791/60607-v

DOI:

10.3791/60607-v

•

04:45 min

February 10, 2022

•

6758 Views

Robert P. Sparks*¹, William Lawless*², Andres S. Arango*^1,3,4, Emad Tajkhorshid^1,3,4, Rutilio A. Fratti^1,3

¹Department of Biochemistry,University of Illinois at Urbana-Champaign ²Department of Chemistry,University of South Florida ³Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign ⁴Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

Summary

Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.