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Journal / Biochemistry / Anvendelse af mikroskala termoforese til måling…
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JoVE Journal Biochemistry
Anvendelse af mikroskala termoforese til måling af protein-lipid interaktioner
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Journal (Biochemistry)
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE
Journal (Biochemistry)
Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE
 
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Anvendelse af mikroskala termoforese til måling af protein-lipid interaktioner

Article DOI: 10.3791/60607-v • 04:45 min • February 10th, 2022
February 10th, 2022 •
*1, *2, *1,3,4, 1,3,4, 1,3
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Chemistry, University of South Florida, 3Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Mikroskala termoforese opnår bindingskonstanter hurtigt til lave materialeomkostninger. Enten mærket eller etiketfri mikroskala termoforese er kommercielt tilgængelig; etiketfri termoforese er imidlertid ikke i stand til mangfoldigheden af interaktionsmålinger, der kan udføres ved hjælp af fluorescerende etiketter. Vi leverer en protokol til mærkede termoforesemålinger.

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Biokemi udgave 180 Fosfoinoitid Phosphatidylinositol 3-phosphat FYVE-domæne Vakuol Lysosom Endosom
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