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Journal / Biochemistry / Användning av mikroskala termofores för att mäta…
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Biochemistry

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JoVE Journal Biochemistry
Användning av mikroskala termofores för att mäta protein-lipidinteraktioner
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Journal (Biochemistry)
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Journal (Biochemistry)
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
 
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Användning av mikroskala termofores för att mäta protein-lipidinteraktioner

Article DOI: 10.3791/60607-v • 04:45 min • February 10th, 2022
February 10th, 2022 •
*1, *2, *1,3,4, 1,3,4, 1,3
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Chemistry, University of South Florida, 3Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Mikroskala termofores erhåller bindande konstanter snabbt till låg materialkostnad. Antingen märkt eller etikettfri termofores i mikroskala är kommersiellt tillgänglig; Etikettfri termofores kan dock inte mångfald av interaktionsmätningar som kan utföras med fluorescerande etiketter. Vi tillhandahåller ett protokoll för märkta termoforesmätningar.

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Biokemi nummer 180 Phosphoinositide Fosfattidylinositol 3-fosfat FYVE-domän Vacuole Lysosome Endosome
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