Journal
/
Cancer Research
/
Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

DOI:
10.3791/60644-v

•

07:53 min

•

March 17, 2020

•

, , ,
1Oncology R&D group,Pfizer Worldwide Research and Development

Chapters

  • 00:05Introduction
  • 00:52Plating TUM622 Cells in the Extracellular Matrix for 3D Culturing
  • 03:523D Coculturing of TUM622 Cells and CAFs (Cancer Associated Fibroblasts) in the Extracellular Matrix
  • 05:39Results: Growth and Morphology
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor phenotype.

Tags

Keywords: 3D CocultureLung Squamous Cell CarcinomaTumor-stroma InteractionsTumor MorphologyTumor ProgressionBasement Membrane MatrixTUM622 CellsCancer-associated FibroblastsDrug Treatment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60644?article_type=v

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
check_url/60644?article_type=v

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
check_url/60644?article_type=v

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
check_url/60644?article_type=v

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
check_url/60644?article_type=v

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
check_url/60644?article_type=v

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
check_url/60644?article_type=v

Acellular and Cellular Lung Model to Study Tumor Metastasis
check_url/60644?article_type=v

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
check_url/60644?article_type=v

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging
check_url/60644?article_type=v

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Read Article