Journal
/
Cancer Research
/
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
JoVE Journal
Cancer Research
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

DOI:
10.3791/60646-v

•

10:27 min

•

July 25, 2020

•

,
1Center for Individualized Medicine,Mayo Clinic, 2Department of Molecular Pharmacology and Experimental Therapeutics,Mayo Clinic

Chapters

  • 00:00Introduction
  • 00:59Selection of Therapeutic Targets
  • 03:45Tumor Engraftment and Maintenance
  • 07:15Testing Responses to Genomically Identified Targets in PDX Models
  • 07:46Representative Results
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to test the efficacy of targeted therapies selected based on the genomic makeup of a tumor. The protocol describes identification and validation of structural DNA rearrangements, engraftment of patients’ tumors into mice and testing responses to corresponding drugs.

Tags

Keywords: CancerTargeted TherapyStructural DNA AlterationPatient-derived XenograftsWhole Genome SequencingGenomic AlterationDruggable ChangesIn Vivo TreatmentTreatment EfficacyAlgorithmMicrosequencingGene SymbolsPathway AnalysisFisher’s Exact TestDruggable GenesGene Cards

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60646?article_type=v

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
check_url/60646?article_type=v

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
check_url/60646?article_type=v

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
check_url/60646?article_type=v

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
check_url/60646?article_type=v

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
check_url/60646?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
check_url/60646?article_type=v

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
check_url/60646?article_type=v

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
check_url/60646?article_type=v

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids
check_url/60646?article_type=v

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Read Article