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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Chapters
- 00:04Title
- 00:41Lipid-based Barcoding
- 01:36Droplet Generation and mRNA Reverse Transcription
- 03:43Post Single Cell Reverse Transcription Cleanup
- 05:18cDNA Amplification
- 06:42Results: Representative Multiplexed Single Cell mRNA Sequencing Analysis
- 07:50Conclusion
Here we presented a multiplexed single cell mRNA sequencing method to profile gene expression in mouse embryonic tissues. The droplet-based single cell mRNA sequencing (scRNA-Seq) method in combination with multiplexing strategies can profile single cells from multiple samples simultaneously, which significantly reduces reagent costs and minimizes experimental batch effects.
