Journal
/
Cancer Research
/
Analysis of Side Population in Solid Tumor Cell Lines
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Analysis of Side Population in Solid Tumor Cell Lines

Analysis of Side Population in Solid Tumor Cell Lines

DOI:
10.3791/60658-v

•

06:26 min

•

February 23, 2021

•

, , , ,
1School of Medicine,Nankai University, 2Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, 3Collaborative Innovation Center for Biotherapy,Nankai University

Chapters

  • 00:04Introduction
  • 00:51Cell Preparation
  • 02:02Cell Staining
  • 03:28Flow Cytometry
  • 04:12Results: SP Analysis
  • 05:54Conclusion

Summary

Automatic Translation

Automatic Translation

A convenient, fast, and cost-effective method to measure the proportion of side population cells in solid tumor cell lines is presented.

Tags

Keywords: Side PopulationCancer Stem CellsTumor Cell LinesHoechst 33342Flow CytometrySP AnalysisStemness PropertiesCell CultureCell Preparation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60658?article_type=v

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
check_url/60658?article_type=v

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
check_url/60658?article_type=v

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
check_url/60658?article_type=v

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
check_url/60658?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
check_url/60658?article_type=v

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
check_url/60658?article_type=v

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
check_url/60658?article_type=v

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone
check_url/60658?article_type=v

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research
check_url/60658?article_type=v

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Read Article