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PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

DOI:
10.3791/60661-v

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15:04 min

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November 02, 2019

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, ,
1Department of Biochemistry,University of British Columbia, 2School of Biochemistry,University of Bristol

Chapters

  • 00:01Title
  • 03:48Protocol
  • 12:19Results
  • 14:19Conclusion

Summary

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We present a method that combines membrane protein purification and reconstitution into peptidiscs in a single chromatographic step. Biotinylated scaffolds are used for direct surface attachment and measurement of protein-ligand interactions via biolayer interferometry.

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Keywords: Membrane ProteinsPeptidiscBiotinylated PeptidiscsProtein Binding AssaysDetergent-freeBio-layer InterferometryFhuAOuter Membrane VesiclesPeptiQuick ReconstitutionNickel-NTA IMACBiotinylated PeptideStreptavidin Coated Sensors

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