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Immunology and Infection
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Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
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Immunology and Infection
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JoVE Journal Immunology and Infection
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

DOI:
10.3791/60680-v

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09:34 min

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March 18, 2020

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, , , ,
1Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP), 2Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol),Universidade Federal de São Paulo (UNIFESP)

Chapters

  • 00:04Introduction
  • 01:31Glial Cells Extraction, Maintenance, and Dissociation
  • 07:28Results: Astrocytes and Microglia Infection
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

The overall goal of this protocol is to instruct how to extract, maintain, and dissociate murine astrocyte and microglia cells from the central nervous system, followed by infection with protozoa parasites.

Tags

Keywords: Astrocyte IsolationMicroglia IsolationGlial Cell ExtractionCentral Nervous SystemImmunological ContextAlzheimer’sParkinson’sProtozoa ParasiteVirus Infection

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