Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Quantifying the Cytotoxicity of Staphylococcus…
JoVE Journal
Immunology and Infection
Author Produced

This content is Open Access.

JoVE Journal Immunology and Infection
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Journal (Immunology and Infection)
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
Journal (Immunology and Infection)
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
 

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Article DOI: 10.3791/60681-v • 12:27 min • January 3rd, 2020
January 3rd, 2020 •
1, 1, 1, 1, 2, 2, 1, 1
1Department of Microbiology and Immunology, Montana State University, 2University Communications, Montana State University

Chapters

Summary
Automatic Translation

This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

Tags

Cytotoxicity Staphylococcus Aureus Polymorphonuclear Leukocytes PMNs Neutrophils Virulence Factors Leukotoxins Host Pathogen Interaction Laboratory Equipment Whole Blood Centrifugation Density Gradients Dextran Sodium Chloride Ficoll Solution Informed Consent
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter