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Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
This content is Free Access.
Chapters
- 00:05Introduction
- 00:39Growth of Blood-Brain Barrier Mimicking Cells
- 02:58Preparation of Bacteria and Cell Treatment
- 03:40Measurement of Permeability
- 04:46Results: Effects of Protein Glycation on the Blood-Brain Barrier
- 05:48Conclusion
The human blood-brain barrier selectively prevents penetration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial traversal.
