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Immunology and Infection
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Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells

Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells

DOI:
10.3791/60692-v

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06:26 min

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February 14, 2020

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, , ,
1Institute of Physiological Chemistry,Martin Luther University Halle-Wittenberg

Chapters

  • 00:05Introduction
  • 00:39Growth of Blood-Brain Barrier Mimicking Cells
  • 02:58Preparation of Bacteria and Cell Treatment
  • 03:40Measurement of Permeability
  • 04:46Results: Effects of Protein Glycation on the Blood-Brain Barrier
  • 05:48Conclusion

Summary

Automatic Translation

Automatic Translation

The human blood-brain barrier selectively prevents penetration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial traversal.

Tags

Keywords: Blood-brain BarrierMicrobial TraversalMicrovascular Endothelial CellsBacterial MeningitisPostoperative DeliriumHigh Throughput AnalysisCollagen IVFibronectinHuman Microvascular Endothelial CellsTrypsin EDTAFCSTrypan BlueE.coli

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