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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

JoVE Journal
Genetics

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JoVE Journal Genetics

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

DOI:

10.3791/60697-v

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10:19 min

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February 03, 2020

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Jinxia Shi, Yijuan Jia, Di Fang, Shidan He, Peng Zhang², Yushuang Guo, Yongli Qiao

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences,Shanghai Normal University, ²College of Agriculture,Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation,Guizhou Institute of Tobacco Science

Chapters

00:04Introduction
00:26Preparation of N. benthamiana 16c Plants
02:08Preparation of Agrobacterium Culture for Infiltration
03:44Co-infiltration N. benthamiana Leaves
04:26GFP Imaging Analysis
04:47Northern Blot Analysis of GFP mRNA Levels in Infiltrated Leaves
08:08Results: Infiltrated N. benthamiana 16c Leaves
09:24Conclusion

Summary

Automatic Translation

Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

Tags

Keywords: RNA Silencing Suppressors Plant Pathogens Screening Assay Nicotiana Benthamiana Systemic RNA Silencing Local RNA Silencing Agrobacterium LB Medium Antibiotics MES Acetosyringone

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