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JoVE Journal
Genetics

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Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
 

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Article doi: 10.3791/60704
February 18th, 2020

Summary February 18th, 2020

This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation buffers.

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