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Genetics

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
 

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Article DOI: 10.3791/60712-v
January 25th, 2020

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Summary January 25th, 2020

3D multicolor DNA FISH represents a tool to visualize multiple genomic loci within 3D preserved nuclei, unambiguously defining their reciprocal interactions and localization within the nuclear space at a single cell level. Here, a step by step protocol is described for a wide spectrum of human primary cells.

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