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Crescita degli strati cellulari endoteliali umani e ovini sulle membrane biomateriali
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
DOI:

05:20 min

February 06, 2020

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Chapters

  • 00:05Introduction
  • 00:35Explant Dissection and Cell Culture Procedure
  • 01:52Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
  • 03:28Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
  • 04:47Conclusion

Summary

Automatic Translation

Questo protocollo descrive i passaggi critici necessari per stabilire e coltivare colture di cellule endoteliali corneali da espianti di tessuto umano o o novino. Viene inoltre presentato un metodo per la subcultura delle cellule endoteliali corneali su biomateriali membranosi.

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