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Crescita degli strati cellulari endoteliali umani e ovini sulle membrane biomateriali
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1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health,Queensland University of Technology, 4Institute of Health and Biomedical Innovation,Queensland University of Technology, 5Science and Engineering Faculty,Queensland University of Technology, 6Faculty of Medicine,University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 8Faculty of Science,University of Western Australia
Chapters
- 00:05Introduction
- 00:35Explant Dissection and Cell Culture Procedure
- 01:52Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
- 03:28Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
- 04:47Conclusion
Questo protocollo descrive i passaggi critici necessari per stabilire e coltivare colture di cellule endoteliali corneali da espianti di tessuto umano o o novino. Viene inoltre presentato un metodo per la subcultura delle cellule endoteliali corneali su biomateriali membranosi.
