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Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography
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Developmental Biology
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JoVE Journal Developmental Biology
Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

DOI:
10.3791/60766-v

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08:22 min

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February 11, 2020

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1Department of Biochemistry and Molecular Genetics, Alcohol Research Center,University of Louisville

Chapters

  • 00:04Introduction
  • 00:58Measuring the Embryonic Volume Using Water Displacement
  • 02:14Treating Embryos with Ethanol
  • 02:50Processing Embryos for Head Space Gas Chromatography
  • 03:58Sample Measurements Using Gas Space Chromatography
  • 05:04Sample Ethanol Peak Integration and Concentration Analysis
  • 06:06Results: Ethanol Levels in Early Embryonic Zebrafish
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

This work describes a protocol to quantify ethanol levels in a zebrafish embryo using head space gas chromatography from proper exposure methods to embryo processing and ethanol analysis.

Tags

Keywords: EthanolZebrafishEmbryosGas ChromatographyPharmacokineticsChorionProteaseVolume MeasurementEmbryo ProcessingTime-course

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