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Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells
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Bioengineering
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JoVE Journal Bioengineering
Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells

Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells

DOI:
10.3791/60769-v

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05:55 min

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February 08, 2020

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1Molecular Physiology and Biophysics Section,National Institute of Neurological Disorders and Stroke, National Institutes of Health

Chapters

  • 00:04Title
  • 00:36Cochlea Dissection
  • 02:06Dextran Labeling and Imaging Preparation
  • 03:52Results: Representative Dextran Labeling and Uptake
  • 05:19Conclusion

Summary

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Here, we present a method for visualizing the uptake of 3 kDa Texas Red-labeled dextran in auditory hair cells with functional mechanotransduction channels. In addition, dextrans of 3–10 kDa can be used to study endocytosis in hair and supporting cells of the organ of Corti.

Tags

Keywords: Dextran LabelingLive Hair CellsMechanotransduction ChannelsCochlear DissectionFluorescent Dextran

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