An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Luz DC. Mart&#237;nez-S&#225;nchez; Rashmita Pradhan; Phuong  A. Ngo; Lena Erkert; Lukas S. Becker; Alastair  J. Watson; Imke Atreya; Markus  F. Neurath; Roc&#237;o L&#243;pez-Posadas

doi:10.3791/60790

Journal

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Immunology and Infection

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An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

5,812 Views

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07:32 min

•

December 03, 2020

DOI:

10.3791/60790-v

DOI:

10.3791/60790-v

•

07:32 min

December 03, 2020

•

5804 Views

Luz DC. Martínez-Sánchez*¹, Rashmita Pradhan*¹, Phuong A. Ngo¹, Lena Erkert¹, Lukas S. Becker¹, Alastair J. Watson², Imke Atreya*¹, Markus F. Neurath*¹, Rocío López-Posadas*¹

¹Medical Clinic 1, University of Erlangen-Nuremberg,University Hospital of Erlangen ²Norwich Medical School,University of East Anglia, Norwich Research Park

Summary

Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.