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An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
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Immunology and Infection
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JoVE Journal Immunology and Infection
An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

DOI:
10.3791/60790-v

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07:32 min

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December 03, 2020

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, , , , , , , ,
1Medical Clinic 1, University of Erlangen-Nuremberg,University Hospital of Erlangen, 2Norwich Medical School,University of East Anglia, Norwich Research Park

Chapters

  • 00:04Introduction
  • 01:07Intravital Microscopy Preparation
  • 02:19Surgical Preparation
  • 03:06Intestinal Mouse Mucosa Staining
  • 03:54Intravital Microscopy Imaging
  • 05:44Results: Representative Epithelial Integrity Analysis
  • 06:51Conclusion

Summary

Automatic Translation

Automatic Translation

Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.

Tags

Keywords: Intravital MicroscopyIntestinal PermeabilityEpithelial Cell SheddingReal-time VisualizationSingle-cell ResolutionPara And Transcellular PermeabilitySurgical PreparationConfocal Laser Scanning MicroscopeImage AcquisitionSequential AcquisitionAnesthetized MouseIntestinal LumenExteriorized Intestinal SegmentIntestinal Mucosa SurfaceAcriflavine Staining

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