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Immunology and Infection

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A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

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Immunology and Infection

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JoVE Journal Immunology and Infection

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

DOI:

10.3791/60806-v

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07:50 min

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March 30, 2020

•

Dinesh Jaishankar, Cormac Cosgrove, Ryan J. Deaton, I. Caroline Le Poole³

¹Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine,Northwestern University, ²Department of Pathology,University of Illinois at Chicago, ³Department of Microbiology and Immunology, Feinberg School of Medicine,Northwestern University

Chapters

00:05Introduction
00:39Antibody and Fluorophore Selecting and Staining
01:52Spectral Library Preparation
02:59Multispectral Imaging
03:54Spectral Unmixing
04:37Analyzing Multispectral Images via Cell Segmentation and Phenotyping
05:52Results: Representative Multispectral Imaging and Cell Quantification
07:07Conclusion

Summary

Automatic Translation

We describe a rapid staining method to perform multispectral imaging on frozen tissues.

Tags

Keywords: Multispectral Fluorescence Imaging Biomarker Detection Co-localization Rapid Staining Method Spectral Library Machine Learning Image Analysis

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