A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

This method is helpful to detect, and co-localize multiple biomarkers in a single cryosection including markers that are not normally detectable in paraffin sections. Using this experimental staining method, the staining time is significantly reduced and the method can be performed in any laboratory. Demonstrating this method will be Dinesh Jaishankar.

He is a skilled postdoc from the lab, and also the Immunotherapy Assessment Core, Facilities Manager. When selecting antibodies for the analysis, consider the excitation and emission filter sets available on the semi-automated imaging system. And after testing various combinations of fluorophore conjugated primary antibodies, prepare fluorophores to be used so that you have minimal spectral overlap as suggested in the table.