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Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
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Bioengineering
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JoVE Journal Bioengineering
Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

DOI:
10.3791/60821-v

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13:53 min

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December 19, 2020

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, , , ,
1Université de Nantes, CNRS, Unité Fonctionnalité et Ingénierie des Protéines (UFIP), UMR 6286

Chapters

  • 00:00Introduction
  • 00:54Synthesis of the UAA: Propargyl-L-Lysine (Prk)
  • 04:46Production of the Recombinant Protein modified by PrK
  • 08:13Removal of the Histidine Tag by TEV Protease Digestion
  • 09:48Assessment of PrK Accessibility and Functionality and Conjugation to the Hapten
  • 11:26Results: Efficient Incorporation of the PrK and Click Conjugation
  • 12:49Conclusion

Summary

Automatic Translation

Automatic Translation

Genetic code expansion is applied for the introduction of an unnatural amino acid bearing a biorthogonal functional group on a carrier protein at a defined site. The biorthogonal function is further used for the site-selective coupling of a carbohydrate antigen to provide a homogeneous glycoconjugate vaccine.

Tags

Keywords: Homogeneous GlycoconjugateVaccineUnnatural Amino AcidClick-chemistryGenetic Code ExpansionCodon SuppressionPropargyl-lysineBioorthogonal Functional GroupN(alpha)Boc-propargyl-lysineSynthetic Oligosaccharide Hapten

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