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Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

JoVE Journal
Genetics

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JoVE Journal Genetics

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

DOI:

10.3791/60832-v

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06:03 min

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June 11, 2020

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Keerat Kaur^2,3, Nishat Sultana^2,3, Yoav Hadas^2,3, Ajit Magadum^2,3, Mohammad Tofael Kabir Sharkar^2,3, Elena Chepurko^2,3, Lior Zangi^2,3

¹Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Chapters

00:04Introduction
01:06Preparation of modRNA Injection
01:32Myocardial Infarction Surgery
04:07Results: Validation of modRNA Delivery
05:20Conclusion

Summary

Automatic Translation

This protocol presents a simple and coherent way to transiently upregulate a gene of interest using modRNA after myocardial infarction in mice.

Tags

Keywords: Modified RNA Gene Transfer Myocardial Infarction Ischemia Reperfusion Transverse Aortic Constriction Intratracheal Intubation LAD Ligation Intramuscular Injection

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