Journal
/
Chemistry
/
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
JoVE Journal
Chemistry
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

DOI:
10.3791/60842-v

•

15:03 min

•

June 16, 2020

•

, , , , , ,
1Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias,Universidade da Coruña, 2Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación,Universidade da Coruña, 3Department of Microbiology and Parasitology, Institute of Aquaculture,Universidade de Santiago de Compostela

Chapters

  • 00:00Introduction
  • 01:06Synthesis of Magnetic Nanoparticles Conjugated with Feroxamine
  • 09:57Bacterial Assay with Y. ENTEROCOLITICA Strains to Quantify the Isolation and Capture of Pathogenic Bacteria with Nanoparticles
  • 13:52Results

Summary

Automatic Translation

Automatic Translation

This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.

Tags

Keywords: Magnetic NanoparticlesSilica CoatingAmine FunctionalizationSiderophore FeroxamineBacteria DetectionIron UptakeNanoparticle SynthesisCore-shell StructureSurface ModificationAnalytical ScienceNanomedicine

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60842?article_type=v

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
check_url/60842?article_type=v

Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay
check_url/60842?article_type=v

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
check_url/60842?article_type=v

Synthesis and Characterization of Functionalized Metal-organic Frameworks
check_url/60842?article_type=v

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
check_url/60842?article_type=v

A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions
check_url/60842?article_type=v

Preparation of Stable Bicyclic Aziridinium Ions and Their Ring-Opening for the Synthesis of Azaheterocycles
check_url/60842?article_type=v

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
check_url/60842?article_type=v

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery
check_url/60842?article_type=v

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Read Article