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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
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JoVE Journal Cancer Research
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

DOI:
10.3791/60850-v

•

08:47 min

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May 01, 2020

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, ,
1p53 Laboratory, Neuros/Immunos,A*STAR (Agency for Science, Technology and Research)

Chapters

  • 00:04Introduction
  • 01:12Cell Culture and Transient Transfection
  • 03:37Compound Preparation
  • 05:06Luciferase Complementation and Viability Assay
  • 06:30Results: PP242 Disrupts eIF4F Complex Formation
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to measure eIF4E-eIF4G interaction in live cells that would enable the user to evaluate drug induced perturbation of eIF4F complex dynamics in screening formats.

Tags

Keywords: EIF4F ComplexEIF4E-eIF4G InteractionProtein-protein Interaction AssayLive Cell MonitoringTranslation RegulationCancer ProliferationPPI InhibitorsHEK 293 CellsTransfectionTrypsinization96-well Plate Seeding

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