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1Department of Obstetrics, Gynecology and Reproductive Sciences, TB Hair Analysis Laboratory,University of California San Francisco, 2Department of Epidemiology and Biostatistics,University of California San Francisco, 3Department of Medicine, Division of HIV, Infectious Diseases, and Global Medicine,University of California San Francisco, 4Department of Medicine, Division of Pulmonary and Critical Care Medicine,University of California San Francisco
Chapters
- 00:04Introduction
- 01:20Hair Sampling
- 02:32Drug Extraction
- 04:18Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) Preparation
- 05:35Data Analysis
- 06:47Results: Representative Drug-Resistant (DR) TB Medication Quantification
- 07:55Conclusion
Current methods of analyzing patients’ adherence to complex drug resistant-tuberculosis (DR-TB) regimens can be inaccurate and resource-intensive. Our method analyzes hair, an easily collected and stored matrix, for concentrations of 11 DR-TB medications. Using LC-MS/MS, we can determine sub-nanogram drug levels that can be utilized to better understand drug adherence.
