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Journal / Developmental Biology / Mikrowaving og fluorophore-tyramid for multipleks …
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Developmental Biology

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JoVE Journal Developmental Biology
Mikrowaving og fluorophore-tyramid for multipleks immunbeising på mus binyrene − Ved hjelp av unconjugert primære antistoffer fra samme vertsarter
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Mikrowaving og fluorophore-tyramid for multipleks immunbeising på mus binyrene − Ved hjelp av unconjugert primære antistoffer fra samme vertsarter

Article DOI: 10.3791/60868-v • 07:37 min • February 21st, 2020
February 21st, 2020 •
1,2, 2, 2, 2
1College of Animal Science & Technology, Henan University of Science and Technology, 2Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University

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Flere ulike metoder er etablert for multipleks immunstaining ved hjelp av primære antistoffer fra samme vertsarter. Her beskriver vi bruken av mikrobølgemediert antistoffstripping og fluorophore-tyramid for å blokkere antistoffkryssreaktivitet under multipleks immunstaining på formalin-fast parafin-innebygde musbinyreseksjoner.

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Utviklingsbiologi Utgave 156 multix immunbeising immunofluorescens tyramid mikrowaving antigengjenfinning antistoffstripping
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