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Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
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Immunology and Infection
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JoVE Journal Immunology and Infection
Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

DOI:
10.3791/60873-v

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13:47 min

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May 19, 2020

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, , ,
1Bacteriology Division,United States Army Medical Research Institute of Infectious Diseases, 2Headquarters Division,United States Army Medical Research Institute of Infectious Diseases, 3Bioqual

Chapters

  • 00:04Introduction
  • 01:21Plating of the Cell Line, RAW 264.7 and Bacterial Culture
  • 06:29FITC-labeling of Bacteria and Bacterial Opsonization
  • 08:46Adherence Assay and Fluorescent Labeling of Mammalian Cells
  • 10:22Microscopy
  • 11:33Results: Representative Micrographs Collected During an OAA Experiment to Evaluate the Efficacy of an Anthrax Vaccine
  • 13:01Conclusion

Summary

Automatic Translation

Automatic Translation

The opsono-adherence assay is an alternative method to the opsono-phagocytic killing assay to evaluate the opsonic functions of antibodies in vaccine development.

Tags

Keywords: Opsono-adherence AssayFunctional AntibodiesVaccine DevelopmentBacillus AnthracisEncapsulated PathogensRAW 264.7 CellsBacillus Anthracis CapsuleBiosafety Level 2India Ink StainingBacterial Suspension

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