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JoVE Journal
Developmental Biology

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Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
 

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Article doi: 10.3791/60888
April 3rd, 2020

Summary April 3rd, 2020

This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.

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