Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Characterization of In Vitro Differentiation of…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Journal (Genetics)
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
Journal (Genetics)
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
 

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Article DOI: 10.3791/60905-v • 07:29 min • May 16th, 2020
May 16th, 2020 •
*1, *1, 2, 1,3
1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands
* These authors contributed equally

Chapters

Summary
Automatic Translation

Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.

Tags

In Vitro Differentiation Human Primary Keratinocytes RNA-Seq Analysis 2D Keratinocyte Differentiation Contact Inhibition Molecular Characterization Epidermal Differentiation Epigenomic Analysis RNA-seq Analysis Pipeline Seeding Density Keratinocyte Growth Medium Proliferation Medium Differentiation Medium Cell Collection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter