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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
 

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Article doi: 10.3791/60909
March 12th, 2020

Summary March 12th, 2020

Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.

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