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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts

Article DOI: 10.3791/60909-v • 10:45 min • March 12th, 2020
March 12th, 2020 •
1, 1, 1,2, 1
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2American Red Cross, National Headquarters

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Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.

Tags

Isolation Characterization Adult Cardiac Fibroblasts Myofibroblasts Technique Flexibility Improvisation Flow-based Isolation Bead-based Isolation Antibodies Target Cells Cardiac Fibroblast Isolation Tissue-specific Fibroblast Isolation Meiling Melzer David Beier Procedure Six-well Plate Dissection Cold KHB 70% Ethanol Ventral Side Appendages Abdominal Skin And Muscle Thorax Ribcage Forceps
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