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Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

DOI:
10.3791/60911-v

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09:03 min

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March 11, 2020

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, , ,
1Department of Pharmaceutical Sciences,University of Maryland Baltimore, 2AIT Bioscience

Chapters

  • 00:04Introduction
  • 00:39In-Cell Fast Photochemical Oxidation of Proteins (IC-FPOP) System Assembly
  • 02:53Cell Preparation
  • 03:44IC-FPOP
  • 05:40Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Analysis
  • 06:21Results: Representative IC-FPOP Characterization
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP).

Tags

Keywords: In-cell FPOPProtein-protein InteractionsProtein Structural ChangesNative EnvironmentsMembrane ProteinsDiseasesCancerGenetic DisordersFlow SystemSyringe PumpLaser IrradiationExcimer LaserCell CultureCell Preparation

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