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JoVE Journal Neuroscience
Geração de Oligodendrocytes e Oligodendrocyte-Conditioned Medium para Experimentos de Cocultura
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Geração de Oligodendrocytes e Oligodendrocyte-Conditioned Medium para Experimentos de Cocultura

Article DOI: 10.3791/60912-v • 09:05 min • February 9th, 2020
February 9th, 2020 •
1, 1,2, 1,3, 1, 1,2, 1
1Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

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Aqui, apresentamos um método eficiente para a purificação de oligodendrocytes e produção de meio condicioso oligodendrocyte que pode ser usado para experimentos de cocultura.

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Neurociência Edição 156 oligodendrocytes meio condicionado fatores secretos cultura celular interações neuro-glia sistema nervoso central rato
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